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GSTA1 Protein (AA 1-222)

This Recombinant GSTA1 protein is expressed in 大肠杆菌(E. Coli).
产品编号 ABIN666922
发货至: 中国

Quick Overview for GSTA1 Protein (AA 1-222) (ABIN666922)

抗原

See all GSTA1 蛋白
GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

蛋白类型

Recombinant

生物活性

Active

宿主

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资源

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大肠杆菌(E. Coli)

应用范围

SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

纯度

> 90 %
  • 产品特性

    AA 1-222

    序列

    MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF

    产品特性

    GSTA1, 1-222aa, Human, E.coli

    纯化方法

    > 90% by SDS-PAGE

    内毒素水平

    < 1 EU per 1ug of protein (determined by LAL method)

    Biological Activity Comment

    Specific activity is > 35,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
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  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT 10 % glycerol

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
  • 抗原

    GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

    别名

    Glutathione S-transferase alpha 1/GSTA1

    背景

    GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

    分子量

    25.6 kDa (222aa) confirmed by MALDI-TOF

    NCBI登录号

    NP_665683
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